5-Lipoxygenase-Dependent Recruitment of Neutrophils and Macrophages by Eotaxin-Stimulated Murine Eosinophils

5-Lipoxygenase-Dependent Recruitment of Neutrophils and Macrophages by Eotaxin-Stimulated Murine Eosinophils

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The roles of eosinophils in antimicrobial defense remain incompletely understood.In ovalbumin-sensitized mice, eosinophils are selectively recruited to the peritoneal cavity by antigen, eotaxin, or leukotriene(LT)B4, a 5-lipoxygenase (5-LO) metabolite.5-LO blockade prevents responses to both antigen and eotaxin.We examined responses to eotaxin in the absence of sensitization and their dependence on 5-LO.

BALB/c or PAS mice and their mutants (5-LO-deficient ALOX; eosinophil-deficient GATA-1) were injected i.p.with eotaxin, eosinophils, or both, and leukocyte accumulation was quantified up to 24 h.Significant recruitment of eosinophils by eotaxin in BALB/c, up to 24 quadruple topical ointment for dogs h, was accompanied by much larger numbers of recruited neutrophils and monocytes/macrophages.

These effects were abolished by eotaxin neutralization and 5-LO-activating protein inhibitor MK886.In ALOX (but not color block iphone case PAS) mice, eotaxin recruitment was abolished for eosinophils and halved for neutrophils.In GATA-1 mutants, eotaxin recruited neither neutrophils nor macrophages.Transfer of eosinophils cultured from bone-marrow of BALB/c donors, or from ALOX donors, into GATA-1 mutant recipients, i.

p., restored eotaxin recruitment of neutrophils and showed that the critical step dependent on 5-LO is the initial recruitment of eosinophils by eotaxin, not the secondary neutrophil accumulation.Eosinophil-dependent recruitment of neutrophils in naive BALB/c mice was associated with increased binding of bacteria.